human pd 1 quantikine elisa kit (R&D Systems)
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human pd 1 quantikine elisa kit
Human Pd 1 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+pd+1+quantikine+elisa/Human+PD-1+Quantikine+ELISA+Kit/pmc12678972-87-7-16
Average 93 stars, based on 19 article reviews
Human Pd 1 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+pd+1+quantikine+elisa/Human+PD-1+Quantikine+ELISA+Kit/pmc12678972-87-7-16
Average 93 stars, based on 19 article reviews
human pd 1 quantikine elisa kit - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Immune regulation of Chidamide-induced Linc01010 accumulation in breast cancer cell death"
Article Title: Immune regulation of Chidamide-induced Linc01010 accumulation in breast cancer cell death
Journal: World Journal of Clinical Oncology
doi: 10.5306/wjco.v16.i11.112514
Figure Legend Snippet: Chidamide induced programmed death-1 expression regulated tumor necrosis factor-related apoptosis-inducing ligand secretion in natural killer cells. A and B: Content of programmed death-1 (PD-1) in the cell lysate; C: An impact on the secretion of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in natural killer (NK) cells. First, a co-culture model was adopted that the breast cancer MCF-7 or MDA-MB-231 cells treated with 20 μM Chidamide were cultured with NK-92 cells for 24 hours, 48 hours and 72 hours respectively, after transfecting with Linc01010 specific small interfering RNA (siRNA). We measured the content of PD-1 in the cell lysate with enzyme-linked immunosorbent assay (ELISA) to screen the best time for Chidamide to induce PD-1 expression. Next, ELISA assay was determined whether programmed death-ligand 1 proteins had an impact on the secretion of TRAIL in NK cells. The mean ± SD for three independent experiments and analyzed using Statistical Package for Social Science software. In A and B: 20 μM Chidamide vs Basal at a P < 0.05, 20 μM Chidamide and Linc01010 siRNA vs 20 μM Chidamide at b P < 0.05. In C: 1000 ng PD-L1 vs 0 ng PD-L1 at a P < 0.05, 500 ng PD-L1 vs 0 ng PD-L1 at b P <0.05, 100 ng PD-L1 vs 0 ng PD-L1 at c P <0.05, 1000 ng PD-1 antibody vs 0 ng PD-L1 at d P <0.05. CHid: Chidamide; NK: Natural killer; PD-L1: Programmed death-ligand 1; PD-1: Programmed death-1; TRAIL: Tumor necrosis factor-related apoptosis-inducing ligand.
Techniques Used: Expressing, Co-Culture Assay, Cell Culture, Small Interfering RNA, Enzyme-linked Immunosorbent Assay, Software
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Recombinant:Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors Article Snippet: Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (Invivogen). .. Serum and recombinant PD-1 was quantified by Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors. Article Snippet: JurkatLucia TCR-hPD-1 cells and Raji-APC-hPD-L1 were added to the wells according to the manufacturer’s directions, and plates were incubated at 37°C for 6 h. Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (InvivoGen). .. Serum PD-1 quantification Serum and recombinant PD-1 were quantified by a Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors Article Snippet: Jurkat-Lucia TCR-hPD-1 cells and Raji-APC-hPD-L1 were added to the wells according to the manufacturer’s directions, and plates were incubated at 37°C for 6 h. Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (InvivoGen). .. Serum and recombinant PD-1 were quantified by a Enzyme-linked Immunosorbent Assay:Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors Article Snippet: Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (Invivogen). .. Serum and recombinant PD-1 was quantified by Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors. Article Snippet: JurkatLucia TCR-hPD-1 cells and Raji-APC-hPD-L1 were added to the wells according to the manufacturer’s directions, and plates were incubated at 37°C for 6 h. Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (InvivoGen). .. Serum PD-1 quantification Serum and recombinant PD-1 were quantified by a Article Title: Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors Article Snippet: Jurkat-Lucia TCR-hPD-1 cells and Raji-APC-hPD-L1 were added to the wells according to the manufacturer’s directions, and plates were incubated at 37°C for 6 h. Supernatants were transferred into a 96-well white plate (VWR) and assayed with QUANTI-Luc 4 reagent (InvivoGen). .. Serum and recombinant PD-1 were quantified by a |